Abstract
The possibility of streamlining general cryoprotection procedures is investigated using aluminium cryoplates with wells, facilitating fluid handling and drying treatment. Precultured shoot tips of potato cultivar ‘Sayaka’ were embedded in calcium alginate gel in wells of the aluminium cryo-plates. In the V cryo-plate protocol, dehydration was performed for 30 min at 25 °C in plant vitrification solution after osmoprotection. In the D cryo-plate protocol, dehydration was performed by placing the cryo-plates for 2.0 h under an air current in a laminar flow cabinet after osmoprotection. In both protocols, cooling was performed by placing the cryo-plates in uncapped cryotubes, which were immersed in liquid nitrogen. For rewarming, the cryo-plates were immersed in liquid MS medium supplemented with 1.0 M sucrose and diluted for 15 min at room temperature. Under these conditions, regrowth rates of cryopreserved shoot tips in V cryo-plate and D cryo-plate were 96.7 and 93.3 %, respectively. Both protocols will facilitate efficient strategies for preservation, storage, and maintenance of genetic stability of important potato germplasm.
Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
