Abstract
Extracts of whole body and of venom sac of wasps (Polistes exclamans) were fractionated by DEAE-cellulose chromatography. Nine fractions were separated from whole body, and eight from venom sac preparations. Fractions 6 of venom sac and 8 of whole body were the active components in direct skin testing. The antigenic heterogeneity of each of the fractionated products was examined by means of appropriate rabbit antisera to wasp extracts. All nine of the whole body fractions gave precipitation, as did four of the eight venom sac fractions. They variously gave between one and three lines of precipitation. In addition to this, fractions 6 and 7 of whole body showed, by testing with rabbit antiserum, common reactions, respectively, with bee and yellow jacket, and were presumed to be the components of wasp that were responsible for cross-sensitizations in patients. A final discussion, comparing the findings for bee, wasp, and yellow jacket, is given in the third paper of this series.
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