Abstract
Abstract It has been shown that when the dialysable fraction (dialysate) of the water soluble extract of ragweed pollen (WSR), which constitutes approximately 95% of the total dry weight of WSR (1), is injected into the skin of ragweed sensitive patients, it will give rise to a positive reaction in all treated cases but induce no reaction in over 80% of those who have had no prior treatment (2). It is therefore of considerable interest to determine the composition of the component(s) which is capable of inducing a skin sensitizing antibody in man. This paper deals with the purification of the allergen(s) in dialysate and its physicochemical properties. Methods and Materials. I. Precipitation of the allergen. Aliquots of varied concentrations of phosphotungstic acid (PTA), picric acid and trichloracetic acid were mixed with 2-ml aliquots of dialysate (20 mg/ml) to determine which of these reagents could precipitate the allergen in as pure a state as possible.
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