Abstract
1. 1. The protein nitrogen content of pollen extracts could be determined directly by analysis of the 10 per cent phosphotungstic acid protein precipitate or indirectly by the difference between the total and nonprotein nitrogen. 2. 2. The activity of pollen extracts, as measured by the protein nitrogen content, varied according to the condition of the pollen and the age of the extract. 3. 3. Deterioration of pollen, measured by the reduction of aqueous soluble protein, was prevented by preserving the dry pollen sealed from atmospheric conditions. 4. 4. The loss of the protein nitrogen and activity in alkaline saline pollen extracts approximated 50 per cent at the end of one year. 5. 5. Repeated filtration of pollen extracts reduced the activity and protein nitrogen content proportionately. The nonprotein nitrogen was not affected. 6. 6. A study of pollen extraction has shown the ready solubility of the active protein material. Long periods of extraction or repeated extractions were not necessary for its solution.
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