The alarmins S100A8 and S100A9 as promising targets for non-invasive molecular imaging of phagocyte activation during inflammation

Abstract

Event Abstract Back to Event The alarmins S100A8 and S100A9 as promising targets for non-invasive molecular imaging of phagocyte activation during inflammation Tom Völler1*, Michel Eisenblätter1, Johannes Roth1 and Thomas Vogl1 1 Institute of Immunology, Germany The proinflammatory alarmins S100A8 and S100A9 are released by activated phagocytes at sites of inflammation. Due to their favorable kinetics S100A8/S100A9 (calprotectin) could be characterized as a very early and sensitive biomarker in a broad spectrum of acute and chronic inflammatory disorders like rheumatoid arthritis, allergies, inflammatory bowel or lung diseases. The purpose of our study was to avail these findings for in vivo imaging of inflammation with the perspective of monitoring disease activity in clinically relevant disorders. S100A9-antibody was coupled to the fluorescence dye Cy5.5 for optical imaging studies by Fluorescence Reflectance Imaging (FRI). We investigated various mouse models of inflammatory and infectious diseases using anti-S100A9-Cy5.5 antibodies. S100A9 knock-out-mice served as additional controls to prove specificity. In the contact dermatitis model injection of anti-S100A9-Cy5.5 resulted in contrast to noise ratios (CNR) which were more than ten-fold higher compared to those of knock-out-mice and also significantly higher after injection of IgG-Cy5.5 without relevant specificity. With this specificity-proven marker we monitored inflammatory models of different immunological etiology, e.g. collagen-induced arthritis (CIA) or experimental leishmaniasis. In both models molecular imaging of S100A9 provides a sensitive and specific method of non-invasive monitoring even in subclinical disease stages. In addition, S100A9 is the first biomarker with a prognostic value for the Th1/Th2-driven inflammatory process in experimental leishmaniasis even weeks prior to the clinical outcome. In conclusion S100A9 has the potential to monitor disease activities in vivo for many other inflammatory diseases associated with a high phagocyte activity. Keywords: S100A8, S100A9, near-infrared optical imaging, biomarkers, experimental leishmaniasis, Clinical Imaging, Prognostic biomarkers, lung inflammation, collagene-induced arthritis Conference: 15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013. Presentation Type: Abstract Topic: Translational immunology and immune intervention Citation: Völler T, Eisenblätter M, Roth J and Vogl T (2013). The alarmins S100A8 and S100A9 as promising targets for non-invasive molecular imaging of phagocyte activation during inflammation. Front. Immunol. Conference Abstract: 15th International Congress of Immunology (ICI). doi: 10.3389/conf.fimmu.2013.02.00333 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 20 Mar 2013; Published Online: 22 Aug 2013. * Correspondence: Mr. Tom Völler, Institute of Immunology, Münster, Germany, tom.voeller@gmx.de Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Tom Völler Michel Eisenblätter Johannes Roth Thomas Vogl Google Tom Völler Michel Eisenblätter Johannes Roth Thomas Vogl Google Scholar Tom Völler Michel Eisenblätter Johannes Roth Thomas Vogl PubMed Tom Völler Michel Eisenblätter Johannes Roth Thomas Vogl Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.