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Abstract
The AJ516 antibody does not detect the human CD1a protein by flow cytometry.
Highlights
CD1a (Uniprot #P06126), a protein displayed at the surface of antigen-presenting cells, is involved in the presentation of lipid antigens to T cells (Hunger et al, 2004)
We describe the inability of the AJ516 antibody, a single chain fragment derived from the 10H3.9 hybridoma, to successfully detect the CD1a protein by flow cytometry in CD1a-transfected HEK293 cells
IgG produced by the mouse hybridoma 10H3.9 detects the CD1a protein at the cell surface of HEK293 transfected cells
Summary
CD1a (Uniprot #P06126), a protein displayed at the surface of antigen-presenting cells, is involved in the presentation of lipid antigens to T cells (Hunger et al, 2004). We describe the inability of the AJ516 antibody, a single chain fragment (scFv) derived from the 10H3.9 hybridoma, to successfully detect the CD1a protein by flow cytometry in CD1a-transfected HEK293 cells. IgG produced by the mouse hybridoma 10H3.9 detects the CD1a protein at the cell surface of HEK293 transfected cells.
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