Abstract
Publisher Summary This chapter discusses the drug-metabolizing enzyme systems, presents the Ah system, and provides data for multiple Ah-structural gene products. A cytosolic receptor is regarded as the major product of the Ah-regulatory genes. Sucrose-density gradient analysis following dextran–charcoal treatment is among the most reliable methods for characterizing an Ah receptor. The aryl hydrocarbon hydroxylase (AHH) fluorescent assay is simple and extremely sensitive. This assay, using benzo[α]pyrene as the substrate in vitro, is most commonly used as the biochemical marker for the Ah locus in laboratory animal and human studies. When males and females of four inbred strains were housed together and allowed to breed randomly for 46 to 48 months, the AHH inducibility by 3-methylcholanthrene of weanlings between 18 and 22 generations approximated the distribution found normally for out-bred or random-bred mouse strains. These data indicate the involvement of multiple Ah regulatory genes and a “natural selection” tendency of the induction process to “drift” toward lower intensity in populations having heterogeneous genetic input.
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