Abstract

A Coulter Counter in conjunction with a pulse height analyser has been used to study the aggregation of two Chinese hamster cell lines over a range of temperature. Pulse height analysis gives a detailed picture of the concentrations and sizes of cell aggregates during the course of aggregation. Aggregates consisting of up to approx. 20 single cells can be detected. The extent of aggregation and the width of the aggregate size distriutions increase with temperature. The modal volumes of the single cells decrease with temperature implying that the larger cells in the single cell population are more adhesive.

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