Abstract
Fructose 6-phosphatase and fructokinase activities were identified in Agaricus bisporus fruit body extracts. These enzymes and mannitol dehydrogenase (MDH; E.C. 1.1.1.138) were assayed during the growth of fruit bodies on compost. MDH activity was high at early stages of development, and declined during growth to very low levels after sporulation. Phosphatase activity rose to a maximum during late fruit body development, and then fell to a low level after sporulation. Fructokinase activity was present throughout development and maximum activity was observed at late development. The results are discussed in relation to previously observed changes in fruit body mannitol content during development.
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