Abstract
SUMMARY The influence of the concentration of sodium chloride on precipitation using chicken antiserum was determined with the infectious laryngo-tracheitis (I.L.T.) anti-I.L.T. system. With less than 2 per cent salt, only high concentrations of reactants could be detected and at least 8 per cent was necessary for detection of threshold amounts of either antigen or antibody. In all further work agar gel with at least 8 per cent salt was used. Using the agar gel double diffusion precipitin test the identity of I.L.T. virus was demonstrated on 40 samples of virus isolated from the field in Great Britain, and 1 from Australia and 2 from New Zealand. Similar tests showed the identity of 5 fowl pox samples. Fowl pox and I.L.T. viruses were differentiated. The egg inoculation and gel diffusion techniques were compared for the diagnosis of I.L.T. and its differentiation from fowl pox on 200 tracheas from fowls dying in over 100 suspected outbreaks of I.L.T. The virus of I.L.T. was isolated by egg inoculation from 150 tracheas and the gel diffusion technique demonstrated I.L.T. precipitating antibody in 112 (75 per cent) of them. In 7 tracheas from which it was not possible to isolate the virus by egg inoculation precipitating antigen was demonstrated by the gel technique.
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