The adrenergic innervation of the rat central amygdaloid nucleus: a light and electron microscopic immunocytochemical study using phenylethanolamine N-methyltransferase as a marker.

Abstract

Using immunocytochemistry of phenylethanolamine N-methyltransferase for light and electron microscopy, investigations were carried out to document the morphology of adrenergic afferents innervating the rat central amygdaloid nucleus and to analyse the manner in which contacts with neurons of the nucleus are formed. With the light microscope, dense terminal plexus of phenylethanolamine N-methyltransferase-immunoreactive axons with typical large boutons (diameter > 1 micron) were found in the medial central nucleus, extending into its ventral subdivision and the adjacent intra-amygdaloid portion of the bed nucleus of the stria terminalis. Electron microscopy of the medial central nucleus showed phenylethanolamine N-methyltransferase-immunoreaction product in the cytoplasm of intervaricose axons and boutons. Large adrenergic boutons contained numerous small clear vesicles and, occasionally, large dense-cored vesicles. In serial sections, most boutons formed synaptic contacts. Synapses of immunoreactive terminals were mainly of the asymmetric type and localized preferentially on medium sized to small dendrites and dendritic spines. Structures postsynaptic to adrenergic boutons were often additionally contacted by non-labelled terminals. The study gives evidence that adrenergic afferents exert a direct synaptic influence on medial central nucleus neurons. The peripheral localization of the majority of adrenergic synapses, their asymmetric configuration, and the presence of non-adrenergic synapsing terminals in their immediate vicinity indicate that the major component of the adrenergic input is of an excitatory nature, and is integrated with innervation from other sources.