The adhesion receptor GPR56 is activated by extracellular matrix collagen III to improve \u03b2-cell function

Oladapo E Olaniru,Peter M Jones,Shanta J Persaud,Attilio Pingitore,Xianhua Piao,Stefanie Giera,Ramón Castañera González

doi:10.1007/s00018-018-2846-4

Abstract

AimsG-protein coupled receptor 56 (GPR56) is the most abundant islet-expressed G-protein coupled receptor, suggesting a potential role in islet function. This study evaluated islet expression of GPR56 and its endogenous ligand collagen III, and their effects on β-cell function.MethodsGPR56 and collagen III expression in mouse and human pancreas sections was determined by fluorescence immunohistochemistry. Effects of collagen III on β-cell proliferation, apoptosis, intracellular calcium ([Ca2+]i) and insulin secretion were determined by cellular BrdU incorporation, caspase 3/7 activities, microfluorimetry and radioimmunoassay, respectively. The role of GPR56 in islet vascularisation and innervation was evaluated by immunohistochemical staining for CD31 and TUJ1, respectively, in pancreases from wildtype (WT) and Gpr56−/− mice, and the requirement of GPR56 for normal glucose homeostasis was determined by glucose tolerance tests in WT and Gpr56−/− mice.ResultsImmunostaining of mouse and human pancreases revealed that GPR56 was expressed by islet β-cells while collagen III was confined to the peri-islet basement membrane and islet capillaries. Collagen III protected β-cells from cytokine-induced apoptosis, triggered increases in [Ca2+]i and potentiated glucose-induced insulin secretion from WT islets but not from Gpr56−/− islets. Deletion of GPR56 did not affect glucose-induced insulin secretion in vitro and it did not impair glucose tolerance in adult mice. GPR56 was not required for normal islet vascularisation or innervation.ConclusionWe have demonstrated that collagen III improves islet function by increasing insulin secretion and protecting against apoptosis. Our data suggest that collagen III may be effective in optimising islet function to improve islet transplantation outcomes, and GPR56 may be a target for the treatment of type 2 diabetes.

Highlights

The extracellular matrix (ECM) is a specialised structure formed from secreted molecules such as proteins, proteoglycans and polysaccharides
Collagen III did not co-localise with insulin in mouse and human islets, suggesting that it was not synthesised within β-cells (Fig. 1a), but it was co-expressed by cells that were immuno-positive for the vascular endothelial marker CD31 (Fig. 1b)
Given that ~ 60% of islet endothelial cells are preserved after 24 h maintenance of islets in culture [32] and RNAs used for RT-PCR were extracted from islets following overnight culture after isolation, it is likely that identification of collagen III mRNA in islet samples (Fig. 1c) and its co-expression with CD31 (Fig. 1b) reflects its expression by islet vascular endothelial cells

Summary

Introduction

The extracellular matrix (ECM) is a specialised structure formed from secreted molecules such as proteins, proteoglycans and polysaccharides. Collagen IV, an abundant protein in islet ECM, promoted adhesion, migration and insulin secretion in studies using human β-cells maintained in culture [11]. Collagen III is a major structural component of the ECM of many tissues and it is present in the pancreas of various mammals, including rodents, pigs and humans [12]. It has been identified as a ligand for an adhesion class G-protein-coupled receptor (GPCR), GPR56 [13], suggesting that it has a signalling role in addition to its structural function. We have investigated whether collagen III exerts paracrine effects by activation of GPR56 to regulate β-cell function

Experimental procedures

Results

F Mouse pancreas

Discussion

H Female AUC

Compliance with ethical standards