Abstract
Intraperitoneal administration of the non-selective adenosine receptor agonist 5’-N-ethylcarboxamide-adenosine (NECA) (0.1 or 0.3 mg/kg) increased fasting serum glucose levels in mice. To clarify the mechanism responsible for this, the expression of liver glucose 6-phosphatase (G6Pase: a gluconeogenic enzyme) was analyzed, and it was found that G6Pase mRNA was increased by NECA treatment. Administration of 0.3 mg/kg NECA resulted in elevated serum glucose levels at 1 h and were further elevated at 6 h. Administration of 0.1 mg/kg NECA increased serum glucose levels at 1 h and had returned to control levels by 6 h. The increase in fasting serum glucose levels induced by NECA are thought to be caused, in part, by elevated G6Pase expression.
Highlights
Adenosine receptor blockade has been linked to the reversal of insulin resistance [1]
While adenosine receptors are involved in NECA-induced insulin resistance during feeding [7,10], there are no reports describing the effects of NECA on gluconeogenesis during fasting
We found that in fasting mice treated with NECA for 6 h, serum glucose levels increased and serum alanine levels decreased [11]
Summary
Adenosine receptor blockade has been linked to the reversal of insulin resistance [1]. The treatment of mice with the non-selective adenosine receptor agonist 5’-N-ethylcarboxamide-adenosine (NECA) increases fasting blood glucose levels and slows glucose clearance, according to glucose tolerance tests [7]. Consistent with expectations, these responses were inhibited by deletion of the A2b receptor [7]. While adenosine receptors are involved in NECA-induced insulin resistance during feeding [7,10], there are no reports describing the effects of NECA on gluconeogenesis during fasting. To clarify the mechanism by which NECA increases serum glucose levels in fasting mice, NECA-. Glucose 6-Phosphatase Expression and Gluconeogenesis induced alterations in liver glucose 6-phosphatase (G6Pase: a gluconeogenic enzyme) expression were analyzed and compared with the effects of NECA on serum glucose levels
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