The Addition of L-Arginine in Capacitation Media to Motility, Viability, and Spermatozoa Capacity of Goats

Abstract

The purpose of this study is improving the provision of animal origin food of in vitro fertilization technology. Specific targets to be achieved in this study is to increase the incidence of capacitation spermatozoan and success in in vitro fertilization process . This research utilized four treatment groups, namely controlled group (P0) is the addition of L- Arginine in spermatozoa (HEPES) capacitance medium , group (PI) is the addition of L- Arginine in spermatozoa (HEPES) capacitation medium by 0,002 M / ml ,group (PII) is the addition of L-Arginine in spermatozoa (HEPES) capacitation medium by 0,004 M / ml. and group (PIII) is the addition of L-Arginine in spermatozoa (HEPES) capacitation medium by 0,006 M / ml Further evaluations included motility, viability, capacitation reaction of spermatozoa. Data obtained in the form of percentage of motility, viability and capacitation reaction is analyzed by F Test. The conclusion of this study, group (PIII) is the addition of L- Arginine in spermatozoa (HEPES) capacitation medium by 0,006 M / ml increase of motility, viability and capacitation reaction Keywords : L-Arginine, capacitation medium, motility, viability and capacitation reaction