Abstract
The lyophilized venom of the spider Agelenopsis aperta (Aranea: Agelenidae) has been re-analyzed by on-line coupled high-performance liquid chromatography and atmospheric-pressure chemical-ionization mass spectrometry (HPLC-UV(DAD)-APCI-MS and MS/MS). Thus, 33 acylpolyamines with 11 different molecular masses, present in various concentrations, were detected, and their structures were disclosed. Different types of possible fragmentation as well as principles of structure elucidation are discussed. The method can be used for rapid separation and analysis of spider-venom constituents.
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