Abstract
Summary The structural requirement of phosphatidyl choline in the inhibition of concanavalin-A (Con A) stimulated blastogenesis in mouse lymphocytes was studied. Lecithin isolated from egg, bovine liver and soy bean were active and that isolated from bovine brain was inactive. Moreover, synthetic dimyristoyl-, dipalmitoyl-, dioleoyl- and 1-palmitoyl-2-oleoyl-phosphatidyl choline were inactive. Egg lecithin was fractionated by high pressure liquid chromatography and the components thus obtained tested for inhibitory activity. Two fractions showing substantial activity were obtained. One contained no detectable phosphorous and it was not further analyzed. The other contained phosphorous and when it was analyzed for fatty acid composition, a high content of palmitate and linoleate was observed. In contrast, other phosphorous containing fractions with low inhibitory activity had none or low linoleate content. Based on these results, linoleate appears to be a crucial structural component for mediating the inhibition of blastogenesis. This hypothesis was confirmed when synthetic dilinoleoyl-phosphatidyl choline was shown to have a higher potency than egg lecithin in the inhibition of blastogenesis.
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