THE ACTIVITY OF HYDROLYTIC ENZYMES IN DIFFERENT LIFE STAGES OF THE HOUSE FLY MUSCA DOMESTICA L.

Abstract

The problem of insect resistance to insecticides attracts great attention in the world. It is known that metabolic resistance in insects is provided by the activity of detoxi-fication enzymes, including hydrolases. Enzyme activities do not remain the same during the life cycle of insects. The purpose of this study was to assess the activity of acetylcholinesterase, alkaline and acid phosphatases in the house fly Musca domes-tica L. at the different lifestages. Enzyme activities were determined in homogenates obtained from eggs, larvae of II – III instar, pupae, and adults (1, 5, and 10 days old) of the M. domestica laboratory strain. According to the obtained results, the acid phosphatase activity did not differ in different life stages of M. domestica, and the activity of acetylcholinesterase and alkaline phosphatase varied in ontogenesis. Alkaline phosphatase activity was minimal in the eggs, increased in the larvae, and decreased again at the pupal stage. The activity of acetylcholinesterase was minimal in the eggs as well and increased with the M. domestica development up to the pupal stage. The acetylcholinesterase activity in one-day-old adults was similar to that in pupal stage.The revealed features of the activity of hydrolytic detoxification enzymes at different life cycle stages of the M. domestica might determine the characteristics of metabolic resistance to insecticides, which, in our opinion, should be taken into account when one studies of insecticide resistance in house flies.