Abstract
Benzyl alcohol achieved a marked activation of the adenylate cyclase activity in a partially purified membrane preparation from rat brain striata, although inhibition resulted at high concentrations. The degree of activation observed depended on the ligand used to stimulate the enzyme, with that observed in the presence of guanosine 5'-[beta,gamma-imido]triphosphate (p[NH]ppG) (5.8-fold)>dopamine+p[NH]ppG (5-fold)> GTP (3-fold)>dopamine+high GTP (2.25-fold)>dopamine (+low GTP)=basal (+low GTP) (1.7-fold). The differences in the concentration-dependence of both the activation and inhibition of dopamine-stimulated and basal activities of the enzyme meant that increasing benzyl alcohol concentrations caused a net elevation in the fold-stimulation of the basal activity by dopamine. Arrhenius plots of p[NH]ppG-, GTP-, fluoride-, dopamine-plus-high GTP- and dopamine-plus-p[NH]ppG-stimulated activities all exhibited a single break occurring at around 22 degrees C. This break point was decreased to around 13 degrees C when 50mm-benzyl alcohol was added to the assays. In the presence of dopamine (+low GTP), Arrhenius plots exhibited two distinct breaks, one at around 21 degrees C and the other at around 11 degrees C. When benzyl alcohol (50mm) was added to these assays of dopamine (+low GTP)-stimulated activity, a single break at around 14 degrees C was observed. For the basal activity the Arrhenius plot exhibited a single break at around 15 degrees C both in the presence and in the absence of 50mm-benzyl alcohol. It is suggested that the enzyme is activated by productive collisions between independent mobile entities and that the activity of the enzyme may be regulated by changes in membrane fluidity. The breaks in the Arrhenius plots of all of the ligand-stimulated activities, but not the basal activity, are attributed to lipid-phase separations occurring in either the inner or the outer halves of the bilayer.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
