The activity of autoreactive IgG antibodies may be blocked by human serum and breast milk immunoglobulins

Abstract

Event Abstract Back to Event The activity of autoreactive IgG antibodies may be blocked by human serum and breast milk immunoglobulins Iglika Djoumerska - Alexieva1, Iliyan Manoylov1, Jordan Dimitrov2 and Andrey Tchorbanov1* 1 Bulgarian Academy of Sciences, Bulgaria 2 INSERM, France Background and objectives In the immune system of healthy individuals can be found B cells that produce IgG antibodies specific to a variety of self antigens. These natural autoreactive antibodies are found in the serum and in the intravenous immunoglobulin preparations. They are able to interact with antigens which are target of some pathological autoantibodies. The auto reactivity of natural IgG antibodies can be induces by extreme environmental conditions during purification (high and low pH values). This activity is not observed when using non-denaturating methods of purification. The in vitro interactions between human serum from a healthy individuals, or pooled human IVIg or breast milk immunoglobulins and human liver antigens were investigated. IgG antibodies were purified by two different methods: immunoaffinity elution (using low pH value buffers) and soft salt precipitation (without extreme value buffers). Based on their molecular mass, the purified IgA and IgM molecules were obtained from the different fractions of serum and colostral proteins. Results Serum or breast milk IgM and IgA isolated by immunoaffinity elution can block the interaction of eluted IgG antibodies or pooled IVIg preparation to human liver antigens. The fraction containing immunoglobulin F(ab')2 fragments has the same ability. Conclusions The results show that binding of auto-reactive IgG antibodies to self-antigens can be inhibited by serum as well as mucosal IgA and IgM. Our study supports previous findings that IgM has a major role in blocking the IgG autoantibodies. We suppose that pooled human IgM and IgA preparations may have a positive immunomodulatory activity in patients with autoimmune diseases. Keywords: Autoimmunity, natural autoantibodies, serum IgG, breast milk, inhibition Conference: 15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013. Presentation Type: Abstract Topic: Translational immunology and immune intervention Citation: Djoumerska - Alexieva I, Manoylov I, Dimitrov J and Tchorbanov A (2013). The activity of autoreactive IgG antibodies may be blocked by human serum and breast milk immunoglobulins. Front. Immunol. Conference Abstract: 15th International Congress of Immunology (ICI). doi: 10.3389/conf.fimmu.2013.02.00630 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 12 Jun 2013; Published Online: 22 Aug 2013. * Correspondence: Dr. Andrey Tchorbanov, Bulgarian Academy of Sciences, Sofia, Bulgaria, tchorban@microbio.bas.bg Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Iglika Djoumerska - Alexieva Iliyan Manoylov Jordan Dimitrov Andrey Tchorbanov Google Iglika Djoumerska - Alexieva Iliyan Manoylov Jordan Dimitrov Andrey Tchorbanov Google Scholar Iglika Djoumerska - Alexieva Iliyan Manoylov Jordan Dimitrov Andrey Tchorbanov PubMed Iglika Djoumerska - Alexieva Iliyan Manoylov Jordan Dimitrov Andrey Tchorbanov Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.