The Activity Characteristics of ABO Blood Group Glycosyltransferases and the Effect of Plasma Preservation on its activity

Abstract

Activities of ABO blood group glycosyltransferases in the plasma of blood donors with different blood groups were detected to discover their normal ranges. In addition, the influence of different plasma storage temperatures and time on the enzyme activity was studied, so as to establish a stable ABO blood group glycosyltransferase activity detection technology system for the auxiliary identification of ABO blood groups. Detect the activities of glycosyltransferase A (GTA) in plasma of type A, AB and O blood donors, and glycosyltransferase B (GTB) in plasma of type B, AB and O blood donors, respectively, to determine the activity range of GTA and GTB in the plasma of normal blood group under this detection technique. The activities of GTA and GTB in plasma of the same ABO blood groups were relatively consistent, while significant difference was found among different ABO blood groups. The activity of GTA was around 27.9±0.3 in plasma of A blood group and 28.3±0.5 in plasma of AB blood group. The activity of GTB in plasma of B blood group was about 24.4±0.5, and that in plasma of AB blood group was about 25.6±0.5. The activities of GTA and GTB in plasma of O blood group were negative. The storage temperature and time of plasma would affect the activities of GTA and GTB. There were no significant changes of the activities of GTA and GTB when the plasma was stored at 4 ℃ for 7 days and -40℃ for 21 days. However, after 28 days of storage at -40 ℃, the activities of GTA and GTB were both decreased significantly. The preservation condition suitable for the detection of ABO glycosyltransferase activity in plasma samples contain short-term storage at 4 ℃ for one week, and cryopreserved at -40 ℃ for no more than three weeks.