Abstract
The effects of various metal ions (Na+, K+, Ca2+, Mg2+, Al3+, Co2+) and anions (Cl–, SO2-4 and CH3COO–) on two cellulases were investigated. Fitting of the data to Michaelis-Menten kinetics showed that Al3+ noncom-petitively inhibited cellulase (Km = 22.68 g/L; Vmax = 0.269 mg/min at 5.0 mmol/L AlCl3) and Mg2+ competitively inhibited cellulase (Km = 50.0 g/L; Vmax = 0.434 mg/min at 10.0 mmol/L MgCl2) Different metal ions increased or decreased inhibition of cellulase activity slightly below 1 mmol/L and strongly over 10 mmol/L. The results indicated that filter paper activeity (FPA) was suitable for analysis of enzymatic saccharification with various lignocellulosic substrates whereas crude cellulase was suitable for applications in the biomass industry. Some metal ions were proved to inhibit cellulase reversibly.
Highlights
Cellulases are widely used in biotechnology and industry [1,2,3]
The results indicated that filter paper activeity (FPA) was suitable for analysis of enzymatic saccharification with various lignocellulosic substrates whereas crude cellulase was suitable for applications in the biomass industry
The effects of these metal ions and anions on crude or purified cellulases were investigated at different concentrations, and enzymatic kinetics parameters were determined to clarify the possible action mechanism
Summary
Cellulases are widely used in biotechnology and industry [1,2,3]. cellulase plays central roles in the bioconversion of renewable cellulosic biomass to commodity chemicals [4,5]. 6.0, 0.05 mol/L to 0.5 mol/L citrate or acetate buffer solution, 40 ̊C to 52 ̊C, solid substrate concentration of 80 to 110 g/L and the final concentrations of 0.5 g/L (NH4)2HPO4, 0.025 g/L MgSO4·7H2O and1.0 g/L yeast extract in the fermenter [3,4,7,8,11].The concentration of ions and chelating agents would play an essential role in the biomass industry when the effect of metal ions and anions on cellulase varied significantly with their concentrations. The effects of these metal ions and anions on crude or purified cellulases were investigated at different concentrations, and enzymatic kinetics parameters were determined to clarify the possible action mechanism
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
