Abstract

The photosystem II (PSII) centers inactive in plastoquinone reduction have been detected in leaves as well as in isolated chloroplasts. An initial variable fluorescence rise from the non-variable fluorescence level F 0 to an intermediate level F i is believed to arise from these inactive centers, and has been widely used as a tool for studying these centers. By numerical simulation, the initial fluorescence rise originating from the active PSII centers is determined. We find that the active centers can make a significant contribution to the fluorescence rise from F 0 to F i. This is independent of light intensity. It thus appears that the use of the initial fluorescence rise to study the inactive centers might not be so straightforward as previously thought.

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