The activation of microorganism inoculum from rumen of beef cattle

Abstract

The aim of this research was to investigate the activation of microorganism inoculum from rumen of beef cattle on lignite coal against number of anaerobic bacteria, the amount of volatile fatty acid (VFA) and biogas production. The research utilized completely randomized design with five treatments of various dosages of microbial inoculum from beef cattle rumen on lignite coal: P1 = 2%; P2 = 4%; P3 = 6%; P4 = 8%; P5 = 10%, and four repetitions. Parameters observed were the amount of VFA, the number of anaerobic bacteria and biogas production on day 2, 5, 10 and 15. Initial activation of beef cattle rumen microorganism inoculum utilized in-vitro technique with MC Dougall’s artificial saliva methods, by using combination of 70% concentrate + 30% forage as beef cattle feed. The rate of biogas production was observed on hour 2, 4, 6, 8, 24, 48 and 72. The highest biogas produced was selected as the optimal hour of inoculum source. The treatment used 250 mL bottle serum, as biogas digester, filled with 100 mL 98-5 media and 12 g lignite coal. Microbial inoculum inoculated in digester using 10 mL syringe and incubated at 39°C. The result showed that the highest rate of biogas production on initial activation was 140 mL at hour 8. The highest average number of anaerobic bacteria was reached in P1, approximately 1068 x 1010 cfu/mL; the highest amount of VFA on day 2 and 5 in P3, accounted by 181 mM (acetate acid 29.76 mM/L; propionate acid 6.93 mM/L; butyrate acid 2.48 mM/L); the highest biogas production was reached on P1, accounted by 37.17 mL (CH4 = 0.68%; CO2 = 99.32%).