Abstract
1. Methods have been developed for the more or less quantitative study of the surface precipitation reaction as it occurs in Arbacia egg protoplasm. 2. Various salts retard or inhibit the first stage of the reaction. 3. The relative effectiveness of the alkaline cations in retarding or inhibiting the reaction is shown by the following series, NH4 〉Na 〉 K 〉Li. 4. Magnesium has a more powerful retarding action than any of the above-mentioned cations. 5. Although calcium is essential for the reaction, higher concentrations of calcium ion have exactly the same effect as other cations in retarding or inhibiting it. 6. The sulphocyanate ion has a greater effect on the reaction than the chloride ion. 7. Various theoretical possibilities are disscussed. The behavior of the reaction toward cations and anions of neutral salts is similar to that of a basic catalysis in homogeneous systems. It is pointed out that there is good evidence that the effective catalyst of the reaction is a lipase.
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