Abstract
Treatment of deoxyribonucleic acid with iodine at pH 5·3 at 98–100° for 5 minutes oxidised the cytosine residues much more rapidly than the thymine residues; guanine and adenine residues were unaffected. Urea was liberated from the oxidised deoxyribonucleic acid on hydrolysis with N-alkali at 100° for 1 hr. Treatment of the oxidised product with a solution of 2% diphenylamine in 66·7% formic acid at 37° for 60 hr. gave thymine-containing oligonucleotides and small amounts of oligonucleotides containing both cytosine and thymine. Thymidine-3′,5′-diphosphate and bisthymidinetriphosphate were isolated and characterised.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: Journal of the Chemical Society. Perkin transactions 1
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
