Abstract
1 The effect of the chaotropic agents on three different preparations of the sarcoplasmic reticulum, i.e. native membranes, membranes whose phospholipids were hydrolyzed by phospholipase A and lipid-deficient membranes has been studied. 2 The chaotropic anions inhibit calcium transport, calcium-dependent ATPase activity, phosphoprotein formation and ATP binding. The effectiveness of the anions increases in the following order: 3 Inhibition occurs immediately and is reversible up to an incubation period of 7 min. 4 The maximal effective concentration of the chaotropic anions (0.4 M) does not solubilize either membrane protein or membrane lipids. 5 The chaotropic anions increase the calcium-permeability of the sarcoplasmic membranes a relatively small amount, so that the permeability increase cannot account for the reduced calcium uptake. 6 The interference between the binding of ATP and chaotropic anions is the most likely mechanism of their action.
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