The action of amytal on frog gastric mucosa

Abstract

The frog gastric mucosa has been shown to be sensitive to amytal. At 2 mM acid secretion was completely inhibited with a rise of resistance, fall in short-circuit current and no significant change in potential difference. Simultaneously there was 75% inhibition of O 2 consumption and 50% depression of ATP levels. Dual-beam spectrophotometric studies of intact mucosa with amytal showed a crossover point between NAD + and FAD. The microsomal NADH oxidase ferricyanide reductase has also been shown to be amytal sensitive. Cl − transport was relatively insensitive to amytal, suggesting a qualitative distinction between the mechanisms underlying the transport of H + and Cl − in the mucosa. This was further brought out by the effects of anoxia in which H + transport was inhibited at 5 min but Cl − transport at minimally 20 min following the onset of anoxia.