Abstract

The effect of 3-(3,4-dichlorophenyl)-1,1-dimethylurea (DCMU) on the relative average oxygen yield per flash, ϕ( t d) as a function of the time t d between the flashes has been investigated in spinach chloroplasts. It was found, that at 2·10 −7 M DCMU — where about 80% of the system II electron transport chains are blocked — the relative average oxygen yield per flash, ϕ( t d), decreases with increasing time t d. This effect shows that DCMU not only acts as an inhibitor of the reducing side of system II, but in addition accelerates the decay of the holes stored in the water-splitting enzyme system Y of photosynthesis.

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