Abstract
Hydrolysis of long-chain triglyceride catalysed by the acid lipase of castor beans proceeds via the intermediate formation of 1,2- and 1,3-diglyceride and 1- and 2-monoglyceride. The 1,3-diglyceride is formed via the reaction sequence: 1,2- Diglyceride ⇌ 1- monoglyceride + fatty acid ⇌ 1,3- diglyceride No indication was found for any direct isomerization of the 1,2-di- and 2-monoglyceride during the reaction. The ester bonds of the 1,2-diglyceride are hydrolysed at similar rates, and the enzyme therefore has no positional specificity. The direct hydrolysis of 1- and 2-monoolein is also catalysed by the enzyme at similar rates.
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