Abstract
The ultraviolet-absorption spectrum of synthetic oligoadenylic acid was studied at different pH values over the range 20–80°. The changes noted were qualitatively similar to those reported for polyadenylic acid. It is inferred from these observations that oligoadenylic acid, like polyadenylic acid, may form stable ordered structures in acidic solutions. The ultraviolet-absorption spectrum of a copolymer of adenylic acid and uridylic acid (AMP/UMP = 1.5) was also found to depend upon pH, and temperature. The changes in the spectrum suggest that in neutral 0.1 M sodium phosphate at 25° the copolymer has an organized secondary structure stabilized by interaction between adenine and uracil residues. In solutions of pH 4.0 or less the spectrum of the copolymer was found to depend on temperature in the manner noted for oligoadenylic acid suggesting that ordered domains, comparable with oligoadenylic acid at the same pH, were formed. The phase diagram deduced for the copolymer was found to be analogous to that reported for the system poly-A plus poly-U.
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Schedule a callMore From: Biochimica et Biophysica Acta (BBA) - Specialized Section on Nucleic Acids and Related Subjects
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