Abstract
A compound, non-acetylatable and diazotizable Bratton-Marshall’s reaction-positive compound, was accumulated in pea seedlings in a “folate-deficient” state induced by soaking the seeds in 2×10−3m sulfonamide, 5-methyl-3-sulfanilamide isoxazole. The compound was isolated and characterized as 5′-phosphoribosyl-5-amino-4-imidazolecarboxamide (AICA-ribotide), known as an intermediate compound in the de novo biosynthesis of inosinic acid in animals and microorganisms. Characterization was based on its paper chromatographic behavior, ultraviolet absorption spectra, infrared absorption spectrum, and the molar ratio of its constituents.Using the isolated AICA-ribotide as substrate, AICA-ribotide formyltransferase (EC 2.1.2.3) was found in extracts of pea seedlings. The enzyme was purified about 40-fold and some of its properties were investigated. Optimum pH was 7.4, and optimum temperature was 37°C. In the enzyme reaction, AICA-ribotide and 10-formyltetrahydrofolate were specifically required as substrates with ...
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