Abstract
BackgroundAmomi fructus is a famous traditional Chinese medicine (TCM) that can exert beneficial effects during the treatment of gastrointestinal diseases and is used widely in China and other countries in Southeast Asia. However, the nonvolatile active ingredients that are present in the water extractions from A. fructus used to treat gastrointestinal diseases have yet to be elucidated. The goal of this study was to identify the nonvolatile active ingredients of A. fructus.MethodsWe used an in situ single-pass intestinal perfusion (SPIP) model to identify the active ingredients of A. fructus that play significant roles in gastrointestinal absorption. In addition, we developed a high-performance liquid chromatography (HPLC) method to identify key fractions in intestinal outflow perfusate.ResultsNineteen components were identified in a water extraction from A. fructus; these exhibited different absorption capabilities in different intestinal segments. Of these, six components were determined by the newly developed HPLC method: catechin, vanillic acid, epicatechin, polydatin, isoquercitrin, and quercitrin.ConclusionsThe current study aimed to identify the active ingredients present in water extractions prepared from A. fructus in a single-intestinal perfusate from rats. Our findings provide an experimental basis to explain the pharmacodynamic actions of A. fructus.
Highlights
Many patients are required to undergo surgery for benign or malignant gastrointestinal lesions every year
We investigated promising nonvolatile components in water extractions prepared from A. fructus in the intestine of rats using the SPIP model and an efficient quantitation method based on high-performance liquid chromatography (HPLC)
Precision The relative standard deviation (RSD) values for peak area response were 0.31% and 0.17%. These figures indicated that the HPLC method showed very good levels of precision for our analysis under the specified conditions
Summary
Many patients are required to undergo surgery for benign or malignant gastrointestinal lesions every year. The mortality and complication rates associated with patients undergoing gastrointestinal surgery have decreased over recent years, mostly due to the development of modern medicine and surgical technology. Conventional forms of western medicine are commonly used to facilitate the treatment of gastrointestinal disease, including fasting, gastric decompression, nutritional support, gastrokinetic agents (e.g., domperidone, cisapride, and duphalac), and anal preparations for promoting defecation (e.g., glycerin and mannitol preparations). While these methods may alleviate clinical symptoms to some extent, they are associated with adverse drug reactions and high costs. The goal of this study was to identify the nonvolatile active ingredients of A. fructus
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