The Abnormality of Spermatozoa Goat after Freezing on -80°C Using Tris Diluent Added Combination Hatching Egg Yolk and Amnion Fluid

Liza Choirun Nisa,Gatot Ciptadi,Sri Rahayu

doi:10.21776/ub.jels.2018.008.03.01

Liza Choirun Nisa, Gatot Ciptadi + Show 1 more

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https://doi.org/10.21776/ub.jels.2018.008.03.01

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Abstract

This study aims to determine the combination of the hatching egg yolk (HEY) and amniotic fluid (AF) in Tris diluent to the type of goat spermatozoa abnormalities after frozen at temperature -80°C. This study used four treatments and 6 replications. The semen is diluted using tris extender and added treatment, there are control group (T 0 ) (Tris egg yolk 20%), T 1 (15% HEY + 5% AF), T 2 (10% HEY + 10% AF), and T3 (5% HEY + 15% AF). After dilution, the semen was equilibrated for 2 hours, then stored using Mr. Frosty and frozen at temperature -80°C in the ultra-freezer for 24 hours. The type of spermatozoa abnormalities type was evaluated on fresh semen and after thawing. The data were analyzed using ANOVA (P<0.05). The results showed that there is no significant difference among treatments. The primary abnormalities in fresh semen, there were macro-cephalic, amorphous, and the secondary spermatozoa abnormalities such as detached head, mid-piece defect, coiled tail, loosehead, shoe-hook tail, and a broken tail. The secondary abnormalities in post-thawing were found both in the control group and treatment, which are detached head, loosehead, coiled tail, shoe-hook tail, and broken tail. The conclusion of this study was the percentage of secondary abnormalities after thawing was increasing both in the control and treatment groups. Keywords: Amniotic Fluid, Hatching Egg Yolk, Mr. Frosty , Type of Spermatozoa Abnormality.

Highlights

The advancement of biotechnology in livestock reproduction is very useful to increase the quality and quantity of livestock
This study aims to determine the abnormalities of goat spermatozoa after freezing at -80°C using tris diluent which added
Hatching Egg yolk and Amniotic Fluid Preparation Egg yolk and amniotic fluid were collected from six chicken eggs aged 8 days

Summary

Introduction

The advancement of biotechnology in livestock reproduction is very useful to increase the quality and quantity of livestock. Reproduction biotechnology that is widely developed, is artificial insemination technique [1]. Artificial insemination generally uses frozen semen from cryopreservation [2]. The availability of liquid nitrogen in some areas cannot be obtained [3], and the price is expensive. To overcome this obstacle, it is necessary to find an alternative way to replace the freezing media by freezing in the ultra-freezer. The freezing and storage of semen in the ultra-freezer have some advantages than using liquid nitrogen. There are large semen storage capacity, shorter equilibration processes, easy sample handling, no need to change the media places regularly, and the cost is cheaper [3]

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