THE ABILITY OF Trichoderma harzianum TO PRODUCE CHITINASE

Abstract

The result showed that optimum temperature for maximum activity of Chitinase was 25 Cº with 2.74 unit/µm/min. while optimum pH for maximum activity Chitinase was 5 with 2.25 unit/µm/min . Significant activities of chitinase was produced by T. harzianum in culture media amended with fresh mycelium of M. phaseolina , where the releasing of reducing sugar N-acetylglucosamin was 280 µg/ml which Significantly differ from T. harzianum culture media amended with fresh and dry mycelium of R.solani and F.solani where the releasing of reducing sugar N-acetylglucosamin was 200 µg/ml. Production of chitinase using a medium containing chitin reached 40 µg/ml . Maximium chitinase inhibition activity, purified by ammonium sulphate, was recorded for M. phaseolina mycelium growth 47.03% , which Significantly differ with of R.solani and F.solani where the inhibition activity was 35.18 and 33.33% respectively