Abstract
DNA replication begins from multiple sites distributed throughout the genome, named replication origins. Despite the increasing amount of data on the properties of replication origins, it is still unknown what factors are the primary determinants of ORC localization. Su(Hw) is a zinc-finger protein responsible for the activity of the best-studied Drosophila insulators. In the present work, we show that the insulator protein Su(Hw) recruits the histon acetyltransferase complex SAGA and chromatin remodeler dSWI/SNF to Su(Hw)-dependent insulators and creates a platform for ORC binding. We have found Su(Hw) to be necessary for chromatin remodeling and ORC recruitment regardless of the surrounding chromatin type. Thus, the global chromatin state does not affect the molecular mechanism underlying ORC positioning in genome; it is rather the DNA-binding proteins that are the key determinants that create the proper chromatin structure for ORC binding. Su(Hw) is the first example of such protein.
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