Abstract
Lysate-platelet rich fibrin (Lysate-PRF) is a scaffold that contains growth factor. Aim: To analyze the ability of two types of Lysate-PRF in inducing the proliferation of fibroblast cells. Material & Methods: Microplate with 24 wells each filled 200 μl suspension DMEM + 10% FBS and 10 × 104 fibroblasts. Then FBS is replaced with a concentration of 1%, for serum starvation process. Each 3 wells were exposed with 50%, 25%, 12.5% Lysate-PRF and 50%, 25%, 12.5% Lysate A-PRF respectively. Three other wells are exposed to 10% FBS. Microplate was incubated for 24 hour at 37°C with 5% CO2. The growth of fibroblast was calculated by automatic cell counter. Results: The highest mean value of the Lysate-PRF group was at 12.5% (312.833) while the Lysate A-PRF group at 25% (303.500). If all the groups compared did not show any significant differences. Conclusions: Lysate-PRF and Lysate A-PRF have the same ability as 10% FBS in inducing fibroblast cell proliferation or the same as physiological condition.
Highlights
Fibroblasts of the pulp are mostly present in the cell-rich zone and can differentiate according to the received signal
Lysate-Platelet rich fibrin (PRF) and Lysate A-PRF have the same ability as 10% FBS in inducing fibroblast cell proliferation or the same as physiological condition
The results showed that the growth factor of PRF can induce the proliferation and differentiation of pulp cells associated with secondary dentin formation [8]
Summary
Fibroblasts of the pulp are mostly present in the cell-rich zone and can differentiate according to the received signal. The odontoblast cell is one of the results of fibroblast cell differentiation; because of this ability, the use of fibroblast in the study may present a biological response [1] [2]. Regeneration is a dynamic process including inflammatory phases, granulation, re-epithelialization and tissue remodeling. Suprastiwii and proteases, so that pulmonary regeneration requires cytokine sources and growth factor [3]
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