The 5′ sequences of VSV in vitro transcription product RNA (± SAM)

Abstract

A variety of different 5′ initiation sequences have been characterized for the in vitro transcription product RNA synthesized by vesicular stomatitis viral transcriptase. In the absence of S-adenosyl-L-methionine (SAM), the predominant 5′ sequences are pppApCp... and G 5′ pppApAp... In the presence of SAM, the predominant 5′ sequences are m 7GpppAmpApCp... and pppApCp...