Abstract
Homo- iso-vanillic acid (3-hydroxy, 4-methoxyphenylacetic acid, iso-HVA was detected in rat urine and brain, with a molar ratio of iso-HVA to HVA of 0·07 in urine and about 0·35 in brain. The free urinary iso-vanyl and vanyl phenolcarboxylic acids were studied after intraperitoneal loads with the following compounds: l-dopa, l -3-O- methyldopa, l -4-O- methyldopa , dopamine, 3-O-methyldopamine, 4- O-methyldopamine and 3,4-dihydroxyphenylacetic acid. The results suggest the following conclusions. (1) The molar ratio iso-HVA/HVA is not constant. After dopa as well as dopamine loads it rises with the increase of the dose of precursor administered, showing that, in vivo, the 4- O-methylation process depends, to some extent, on the substrate concentration. (2) In contrast with the other catechols (dopamine and 3,4-dihydroxyphenylacetic acid), l-dopa itself does not seem to be para- O-methylated. It is therefore unlikely that 4- O-methyldopa would be a metabolite of l-dopa in vivo. (3) Distinct urinary metabolites from l -3-O- methyldopa (vanylmandelic and vanillic acids) on one hand and from l-4-O-methyldopa (unknown iso-vanyl phenolcarboxylic acid) on the other, support evidence that some of the metabolic transformations of the side-chain of the O-methyl-catecholamines are different according to whether the methyl group is bound on the meta or on the para position. (4) The high level of cerebral iso-HVA might be due to either a lower iso-HVA than HVA transport outside the brain, or to the existence, in addition to the dopamine source, of a second cerebral metabolic pathway for the production of iso-HVA.
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