Abstract
The International Antibody Validation meetings offer a welcome British forum for discussing this important topic, which is existentially crucial for the biological sciences community. Now in its 6th year, the biennial meeting is organized by Andrew Chalmers (University of Bath; CiteAb), this year with Carly Dix (Astra Zeneca). The organizers gathered some 100 members of industry and academia, producers and users, for a day and a half to describe their efforts to ensure that their antibodies have the desired specificity and selectively for well-defined molecular targets. The meeting is largely available as WebCasts ( http://www.antibodyvalidation.co.uk/past-events/2018).
Highlights
The International Antibody Validation meetings offer a welcome British forum for discussing this important topic, which is existentially crucial for the biological sciences community
Jason reminded the meeting that the specificity of antibody binding is dependent on concentration; higher concentration permits binding to lower affinity epitopes
Since 2015 Abcam moved to antibody validation at scale using CRISPR-Cas[9] target knock-out cell lines. Such knock-out cell lines are increasingly commercially available, and specific loss of signal following knock-out is a robust way of demonstrating antibody specificity for a target in many assay formats[13]
Summary
The International Antibody Validation meetings offer a welcome British forum for discussing this important topic, which is existentially crucial for the biological sciences community. He emphasized the need for perfect and appropriately glycosylated antigens for immunization and validation, and that titration of reagents is crucial to optimize signal-to-noise in the particular experimental context in use. Since 2015 Abcam moved to antibody validation at scale using CRISPR-Cas[9] target knock-out cell lines.
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