Abstract
BackgroundThe 3D8 single chain variable fragment (scFv) is a mini-antibody sequence that exhibits independent nuclease activity against all types of nucleic acids. In this research, crossing a 3D8 scFv G1 transgenic rooster with wild-type hens produced 3D8 scFv G2 transgenic chickens to evaluate suppression of viral transmission.ResultThe transgenic chickens were identified using genomic PCR and immunohistochemistry. To evaluate Newcastle disease virus (NDV) protection conferred by 3D8 scFv expression, transgenic, non-transgenic, and specific pathogen-free (SPF) chickens were challenged with virulent NDV by direct injection or aerosol exposure. The three groups of chickens showed no significant differences (p < 0.05) in mean death time after being directly challenged with NDV; however, in contrast to chickens in the non-transgenic and SPF groups, chickens in the transgenic group survived after aerosol exposure. Although the transgenic chickens did not survive after direct challenge, we found that the chickens expressing the 3D8 scFv survived aerosol exposure to NDV.ConclusionsOur finding suggest that the 3D8 scFv could be a useful tool to prevent chickens from spreading NDV and control virus transmission.
Highlights
The 3D8 single chain variable fragment is a mini-antibody sequence that exhibits independent nuclease activity against all types of nucleic acids
G2 transgenic chickens were generated by breeding a G1 transgenic rooster and wild-type hens for experiments on antiviral treatments against Newcastle disease virus (NDV)
3D8 Single chain variable fragment (scFv) expression in transgenic and wild-type chicken tracheal tissues was identified by immunohistochemical staining
Summary
The 3D8 single chain variable fragment (scFv) is a mini-antibody sequence that exhibits independent nuclease activity against all types of nucleic acids. To evaluate Newcastle disease virus (NDV) protection conferred by 3D8 scFv expression, transgenic, non-transgenic, and specific pathogen-free (SPF) chickens were challenged with virulent NDV by direct injection or aerosol exposure. The transgenic chickens did not survive after direct challenge, we found that the chickens expressing the 3D8 scFv survived aerosol exposure to NDV. Earlier data from oropharyngeal and cloacal swabs have shown that 3D8 scFv transgenic chickens in a contact delivery group exhibited viral clearance after inoculation with an avian influenza virus [14]. The antiviral effect of the 3D8 scFv was evaluated via direct challenge and aerosol exposure to the virulent Kr005 strain of NDV
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
