The -173G ＞C polymorphism in the MIF gene and bladder cancer risk

Abstract

Objective To investigate the association between the - 173G ＞ C polymorphism in the macrophage migration inhibitory factor (MIF) gene and bladder cancer risk in a southern Chinese pop-ulation. Methods The polymorphism was analyzed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique using genomic DNA isolated from 217 bladder cancer pa-tients,and 249 age-and sex-matched controls. Stratification analysis by age, sex, smoking status, and alco-hol status was performed. Results Compared with the - 173GG genotype,individuals with GC/CC geno-types had a significantly decreased risk of bladder cancer (OR = 0. 63,95% CI = 0.43-0.93). The strati-fication analysis revealed that the decreased risk was more pronounced among older subjects (0.58,0.38-0. 89) ,men (0.51,0.33-0.78) ,smokers (0.50,0.29-0.86) ,and drinkers (0.40,0.23-0.70). Conclu-sion Our data suggest that MIF-173G ＞ C polymorphism may contribute to the etiology of bladder cancer in a southern Chinese population. Key words: MIF; Polymorphism; Bladder carcinoma; Molecular epidemiolagy