Abstract
RNA was extracted from human gestational membranes and villous placental tissue following spontaneous delivery (n=15) or elective caesarean section (n=15) at term. The samples were subjected to Northern analysis, using a 2kb cDNA probe for peroxisome proliferator activated receptor (PPAR)-γ. The mRNA was detectable in all choriodecidual and villous placental samples, irrespective of mode of delivery, but was only rarely detectable in the amnion. The JEG3 choriocarcinoma cell line also expressed PPARγ. In order to evaluate PPAR mediated transcriptional activation in JEG3 cells, the cells were transfected with pTK-PPREx3-luc, a PPAR response element (PPRE) containing luciferase reporter construct. Subsequent treatment with 10μm 15-deoxy-Δ12,14prostaglandin J2(15dPGJ2) resulted in an eight-fold stimulation of luciferase production relative to controls transfected with the same construct lacking the PPRE. This stimulation was concentration-dependent. These results suggest roles for PPARγ and its ligand in lipid, steroid and inflammatory mediator homeostasis and in remodelling of gestational tissues.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
