Abstract
We determined the nucleotide sequence of a 4599-bp DNA genomic fragment including the γ-actin encoding gene from Blakeslea trispora, showing an open reading frame of 1561 bp interrupted by four introns with fungal consensus splice-site junctions. The untranslated regions of the actA gene contain a consensus TATA box, a CCAAT motif, a large pyrimidine stretch, and the polyadenylation sequence AATAAA. The predicted protein (375 amino acids) revealed high identity to γ-actins from fungi (>90%), and gene phylogenies support the grouping of B. trispora actin close to those from the majority of the filamentous fungi. actA transcript (1.4 kb) level in β-carotene producing conditions was faintly higher than carRA (1.9 kb) and slightly lower than carB (1.8 kb) β-carotene biosynthetic genes. The use of the actA promoter (P actA) for heterologous gene expression was ascertained by the transformation of gene fusions with the bleomycin resistance gene ( ble R) from Streptoalloteichus hindustanus and the geneticin resistance marker ( aphI) from Tn903, into Escherichia coli and Acremonium chrysogenum.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
