Abstract
Thaumatin-like proteins, which belong to the pathogenesis-related protein 5 family, are an important part of plant defence responses. In this study, five full-length cDNAs encoding thaumatin-like protein (PnTLP1–PnTLP5) were isolated from Panax notoginseng by rapid amplification of cDNA ends. A quantitative real-time PCR analysis indicated that the PnTLP expression levels were regulated by ethylene, salicylic acid, methyl jasmonate, and H2O2. Additionally, the PnTLP expression levels were up-regulated by an Alternaria panax infection, peaking at 4 h post-inoculation. Moreover, the PnTLP2-GFP fusion protein was localised in the cell wall of onion inner epidermal cells. Furthermore, the recombinant PnTLP2 protein produced in Escherichia coli exhibited antifungal activities against A.panax and A. alternata. Similarly, the overexpression of PnTLP2 in transgenic tobacco enhanced the resistance to A. panax. These results suggest that PnTLP genes are defence-related genes expressed in P. notoginseng in response to an A. panax infection.
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