Abstract
A Gram-stain-negative, facultatively anaerobic, motile and rod-shaped bacterium, designated D20T, was isolated from the saline Lake Dai in Inner Mongolia, PR China. Growth of strain D20T occurred at 25-45 °C (optimum, 40 °C), pH 4.0-12.0 (optimum, 8.0) and with 0-3 % NaCl (w/v); (optimum, 0-1 %). The results of 16S rRNA gene sequence analysis revealed that strain D20T was most closely related to three Thauera species, Thaueraselenatis AXT, Thaueraaminoaromatica S2T and Thaueraaromatica K172T, with a similarity value of 96.2 %. The major respiratory quinone of strain D20T was ubiquinone-8 (Q-8), and the dominant fatty acids (>10 %) were summed feature 3 (C16 : 1ω6c and/or C16 : 1ω7c; 39.8 %), C16 : 0 (30.9 %) and summed feature 8 (C18 : 1ω6c and/or C18 : 1ω7c; 13.5 %). The polar lipid profile contained phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol, one aminophospholipid and five unidentified lipids. The DNA G+C content was 67.2 mol% (data from the genome sequence). The estimated genome size was 3.7 Mb. The phenotypic, genotypic and chemotaxonomic differences between strain D20T and its phylogenetic relatives indicated that strain D20T should be regarded as a novel species in the genus Thauera, for which the name Thaueralacus sp. nov. is proposed. The type strain is D20T (=MCCC 1H00305T=KCTC 62586T).
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have