Thallium flux assay adaptation for multi-instrument compatibility.

Abstract

Over the past 15+ years, fluorescence-based measures of Tl+ flux have brought about the discovery of small-molecule modulators of a host of ion channels, transporters, GPCRs and other targets of interest for both drug discovery and basic research. Here, we introduce ION's Brilliant Thallium Snapshot Assay Kit, which provides a brand new way to conduct thallium flux assays. Brilliant Thallium Snapshot is designed for multi-well plate-based, high-throughput measurements of Tl+ flux through K+, Na+, non-selective cation channels, and some Na+ or K+ transporters. Unlike traditional thallium flux assays, our patent-pending Snapshot assay format generates a long-lasting signal so you can easily detect and quantify changes in ion channel or transporter activity on most fluorescence-capable instruments. So whether you have a FLIPR (HTS), a fluorescence microscope, a standard fluorescence plate reader, a high-content imager (HCS), or a flow cytometer - you can run functional screens on viable cells using thallium flux, enabling entirely new possibilities.