Thai plants with high antioxidant levels, free radical scavenging activity, anti-tyrosinase and anti-collagenase activity

Moragot Chatatikun,Anchalee Chiabchalard

doi:10.1186/s12906-017-1994-7

Moragot Chatatikun, Anchalee Chiabchalard

Open Access

https://doi.org/10.1186/s12906-017-1994-7

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Abstract

BackgroundUltraviolet radiation from sunlight induces overproduction of reactive oxygen species (ROS) resulting in skin photoaging and hyperpigmentation disorders. Novel whitening and anti-wrinkle compounds from natural products have recently become of increasing interest. The purpose of this study was to find products that reduce ROS in 14 Thai plant extracts.MethodsTo determine total phenolic and flavonoid content, antioxidant activity, anti-tyrosinase activity and anti-collagenase activity, we compared extracts of 14 Thai plants prepared using different solvents (petroleum ether, dichloromethane and ethanol). Antioxidant activities were determined by DPPH and ABTS assays.ResultsTotal phenolic content of the 14 Thai plants extracts was found at the highest levels in ethanol followed by dichloromethane and petroleum ether extracts, respectively, while flavonoid content was normally found in the dichloromethane fraction. Scavenging activity ranged from 7 to 99% scavenging as assessed by DPPH and ABTS assays. The ethanol leaf extract of Ardisia elliptica Thunb. had the highest phenolic content, antioxidant activity and collagenase inhibition, while Cassia alata (L.) Roxb. extract had the richest flavonoid content. Interestingly, three plants extracts, which were the ethanolic fractions of Annona squamosa L., Ardisia elliptica Thunb. and Senna alata (L.) Roxb., had high antioxidant content and activity, and significantly inhibited both tyrosinase and collagenase.ConclusionOur finding show that the ethanol fractions of Annona squamosa L., Ardisia elliptica Thunb. and Senna alata (L.) Roxb. show promise as potential ingredients for cosmetic products such as anti-wrinkle agents and skin whitening products.

Highlights

Ultraviolet radiation from sunlight induces overproduction of reactive oxygen species (ROS) resulting in skin photoaging and hyperpigmentation disorders
Flavonoids were not found in the petroleum ether and dichloromethane extracts from Stemona curtisii Hook.f., and petroleum ether extracts from Streblus asper Lour. and Phyllanthus acidus (L.) Skeels
Total flavonoid content did not correlate with total phenolic content (R2 = 0.0284, Fig. 1a)

Summary

Introduction

Ultraviolet radiation from sunlight induces overproduction of reactive oxygen species (ROS) resulting in skin photoaging and hyperpigmentation disorders. Novel whitening and anti-wrinkle compounds from natural products have recently become of increasing interest. The purpose of this study was to find products that reduce ROS in 14 Thai plant extracts. Overexposure to sunlight, in particular UVA and UVB, induces the overexpression of reactive oxygen species (ROS) which damage lipids, proteins and deoxyribonucleic acids. Excessive ROS increases expression of collagenase, a protease that degrades collagen which can result in Chatatikun and Chiabchalard BMC Complementary and Alternative Medicine (2017) 17:487 irritation and bacterial infection [4]. Researchers have focused on natural products that inhibit UV-induced ROS, suppress enzymes, and reduce melanin formation as alternatives to current treatments. Plants may reduce wrinkle formation and hyperpigmentation caused by sunlight exposure

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