Abstract
BackgroundHair follicle morphogenesis is orchestrated by bidirectional ectodermal–mesenchymal signaling. In particular, TGFβ (transforming growth factor-beta) family is an important regulator of hair follicle formation and cycling. In this study, we investigated the effect of TGFβ family mimetic peptide (dermal papilla stem cell activator-1, DPS-1) on activation of hair follicle formation.MethodsCell viability, cell cycle, and mRNA expression of human dermal papilla (HDP) cells after DPS-1 or vehicle treatment were determined, and histological analysis of hair cycle was performed from the mouse dorsal back skin after DPS-1 or vehicle administration.ResultsDPS-1 promotes proliferation from 3D sphered HDP cells, but not from 2D cultured HDP cells. DPS-1 induces mRNA expression of genes, which are responsible for hair growth from HDP cells. Moreover, topical administrated DPS-1 stimulates hair growth in mice. Subsequently, histological analysis showed that the diameter and depth of hair follicles were remarkably higher in mice that were administered with DPS-1 compared to vehicle control.ConclusionsDPS-1 promotes proliferation of HDP cells as well as hair growth partly via activation of WNT/β-catenin signaling.
Highlights
Hair follicle morphogenesis is orchestrated by bidirectional ectodermal–mesenchymal signaling
Because transforming growth factor β (TGFβ) family is crucial for hair follicle (HF) morphogenesis as well as hair cycle, we developed synthetic peptides which are structurally similar to endogenous human TGFβ family but possess unique sequences, which we named, dermal papilla stem cell activator-1 (DPS-1)
Effect of DPS-1 on human dermal papilla (HDP) cell proliferation Because of previous report that TGFβ family activates hair cycle by promoting HDP activation, we determined whether DPS-1 promotes HDP growth
Summary
Hair follicle morphogenesis is orchestrated by bidirectional ectodermal–mesenchymal signaling. TGFβ (transforming growth factor-beta) family is an important regulator of hair follicle formation and cycling. We investigated the effect of TGFβ family mimetic peptide (dermal papilla stem cell activator-1, DPS-1) on activation of hair follicle formation. Each stage of hair cycle is regulated by signaling between the epithelium and mesenchymal cells that are located at the base of HF (Ehama et al 2007). BMP from transforming growth factor β (TGFβ) superfamily is important for HF development (Millar 2002; Botchkarev and Paus 2003; Rendl et al 2008). Previous studies showed that WNT/β-catenin and TGFβ family interact to regulate the transcription of a number of genes, which is related with HF formation. There are synergistic effects between WNT/β-catenin and TGFβ signaling pathway in various cellular functions
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