烧伤后增生性瘢痕患者血清中TGF-β、VEGF的浓度检测及意义

Abstract

目的：探讨烧伤后增生性瘢痕患者血清中TGF-β、VEGF浓度变化及意义。方法：选择2009，8~2011，8我科住院的烧伤后增生性瘢痕患者共74例，根据创面愈合后瘢痕增生时间长短分组，分为4组，选择12例正常人作为正常对照组，共5组。用酶联吸附法(ELISA)检测患者血清中TGF-β、VEGF的浓度，5组之间统计比较分析。结果：瘢痕患者血清中TGF-β、VEGF含量与瘢痕增生时段关系密切，1~3月早期瘢痕组血清中TGF-β、VEGF含量开始增加，随瘢痕的发展升高，4~6个月瘢痕组血清中TGF-β、VEGF含量达到了高峰。随着瘢痕成熟，TGF-β、VEGF浓度又逐渐下降。结论：烧伤后增生性患者血清中TGF-β、VEGF的含量可较好地反映增生性瘢痕代谢活跃程度，是增生性瘢痕增生活跃程度敏感的标记物。 Objective: To explore the dose and its clinic significance of profibrotic cytokines of TGF-β and VEGF in the serum of hypertrophic scar patients at excessive stages. Methods: Serum samples of 74 inpatients were collected, who were admitted to our hospital between 2009, 8 and 2011, 8 and were suffering from post-burned hypertrophic scars at various stages. Serum samples of 12 cases of healthy persons were also collected as the control group. Hypertrophic scar groups were divided into 4 subgroups according to the phase of scars and detected the concentrations of TGF-β and VEGF in serum of the trials using the ELISA method. Results: The doses of TGF-β and VEGF began to increase in 1 to 3 months hypertrophic scar group and achieved summit concentration in 4 to 6 months scar group. The concentration of TGF-β and VEGF degreased gradually with the maturation of hypertrophic scars. Conclusions: High concentration of TGF-β and VEGF in the serum can reflect the level of metabolic activity in the tissue of hypertrophic scars. TGF-β and VEGF may be a promising marker in discerning the growth and development of hypertrophic scars.