Texture analysis of protein distribution images to find differences due to aging and superfusion.

Abstract

Many pathologies are age-related, e.g., cardiovascular disease generally occurs in midlife and cancer later in life. This suggests that aging predisposes the body to pathology. Plasma protein spatial distribution images of rat mesentery extracellular matrix (ECM) show texture due to the ECM structures, and there is an age-related decrease in tissue protein that may be related to matrix structure changes. The objective of this study was to compare changes in protein image texture under two conditions: superfusion with normal saline solution and aging. The decrease in soluble protein concentration during superfusion is a washout process, while the mechanism for the age-related decrease in tissue protein is unknown. Therefore, effects of aging and superfusion on tissue protein image texture were compared. Spatial co-occurrence matrix and Fourier analysis techniques have been used for texture evaluation. Superfused images showed a more uniform protein texture. There were gradual age-related changes in image texture parameters. Entropy increased with age from 140 to 630 days, indicating that protein distribution became more disorganized. The results suggest that changes in protein image texture are due to age-related alterations in matrix structure because removing only protein by superfusion had opposite effects on texture parameters.